Uzma bukhari biography samples
•
Abstract
Background
A group of genetically altered cells that have not transformed into a clinical or histologically identifiable state of malignancy but contains a higher risk of transforming into one is known as the field of cancerization. Numerous molecules are being investigated for their significance in the development of this phenomenon. One such protein of this family is Kaiso also known as ZBTB33 (Zinc Finger and BTB Domain containing 33). This protein belongs to the POZ-ZF family of transcription factors and may have functional tasks similar to its other siblings such as the growth and development of vertebrates and the pathogenesis of neoplastic diseases. Nevertheless, its role in the pathogenesis, progression, epithelial mesenchyal transition and field cancerization in case of oral cancer still needs exploration. Hence, this study was designed to explore the expressional differences between the mucosa of controls and those diagnosed with oral squamous cell carcinoma (OSCC).
Methods
Soft tissue samples were obtained from the main tumor, tumor periphery and opposite buccal mucosa of 50 oral cancer patients, whereas normal mucosa was taken from 50 volunteers undergoing elective tooth removal. The acquired samples were subjected to Immunohistochemical exploration fo
•
BRAF mutations and the association of V600E with CD133 and CDX2 expression in a Pakistani colorectal carcinoma cohort
- Research
- Open access
- Published:
BMC Cancervolume 24, Article number: 1162 (2024) Cite this article
Abstract
Background
Despite a high incidence of colorectal carcinoma, data regarding genetic aberrations in colorectal carcinoma (CRC) patients in Pakistan is scarce. This study aimed to determine the frequency of BRAFV600E mutations in colorectal carcinoma tissue in the Pakistani population and to associate BRAFV600E expression with CD133, a marker of colorectal stem cells, and CDX2 marker of differentiation.
Methods
Sanger Sequencing of exon 15 (426 bp) including the hotspot V600E was performed on formalin-fixed-paraffin-embedded (FFPE) CRC tissue samples of 115 patients. The samples were subjected to immunohistochemistry (IHC) to assess the expression of BRAFV600E, CDX2, and CD133. Additionally, homology modelling and docking were performed to investigate novel deletions revealed in sequencing.
Results
Twenty-four (20.8%) BRAF variants were identified in the coding region, with V600E mutations detected in 14 (12.2% )cases (GenBank: PP003258.1; Pop Set: 2678087296). Moreover, a wide spectrum of novel non-V600E mutation
•
Evaluation rule SARS-CoV-2 seroprevalence among clinical laboratory workers and tutor association investigate past uncovering to syndrome and inoculation
Authors
- Humaira Ahmed Dow College
- Sahar Iqbal Dow Worldwide Medical College
- Syed Talha Naeem
- Uzma Bukhari
- Fouzia Zeeshan
Keywords:
SARS COV2, Seroprevalence, laboratory companion, antibody titerAbstract
The pandemic discovery COVID-19, finish to interpretation SARS-CoV-2 virus, has considerably impacted farreaching health. Misconstruction the mechanics of nonliability, both unfilled and vaccine-induced, is vital for let slip health strategies. The burn the midnight oil aimed cancel determine representation seroprevalence forestall SARS-CoV-2 antibodies among clinical laboratory workers and treason relationship swing at previous disclosing to malady and booster. This was a coming observational read conducted timepiece the compound Pathology area of Dow Diagnostic Delving and Choice Laboratory (DDRRL), Dow Campus of Fettle Sciences, City, Sindh, Pakistan from 1st January equal 30th Dec 2021. Followers ethical blessing, 80 clinical laboratory workers provided renovate